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Effect of polymer charge on functional reconstitution of membrane proteins in polymer nanodiscs

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Abstract

Although there is a growing interest in using polymer lipid-nanodiscs, the polymer charge poses limitations for studies on membrane proteins. Here, we demonstrate the functional reconstitution of a large soluble-domain containing positively-charged ∼57 kDa cytochrome-P450 and negatively-charged ∼16 kDa cytochrome-b5 in lipid-nanodiscs, and the role of the polymer charge for high-resolution studies on membrane proteins.

Graphical abstract: Effect of polymer charge on functional reconstitution of membrane proteins in polymer nanodiscs

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Publication details

The article was received on 25 May 2018, accepted on 03 Aug 2018 and first published on 03 Aug 2018


Article type: Communication
DOI: 10.1039/C8CC04184A
Citation: Chem. Commun., 2018, Advance Article
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    Effect of polymer charge on functional reconstitution of membrane proteins in polymer nanodiscs

    T. Ravula, N. Z. Hardin, J. Bai, S. Im, L. Waskell and A. Ramamoorthy, Chem. Commun., 2018, Advance Article , DOI: 10.1039/C8CC04184A

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