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Label-free and high-throughput bioluminescence detection of uracil-DNA glycosylase in cancer cells through tricyclic cascade signal amplification

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Abstract

We develop a label-free and high-throughput bioluminescence method for the sensitive detection of uracil DNA glycosylase (UDG) through enzyme-mediated tricyclic cascade signal amplification. This method exhibits high sensitivity with a detection limit as low as 0.00031 U mL−1, and it can be further applied for the measurement of enzyme kinetic parameters and the screening of UDG inhibitors as well as cancer cell analysis.

Graphical abstract: Label-free and high-throughput bioluminescence detection of uracil-DNA glycosylase in cancer cells through tricyclic cascade signal amplification

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Publication details

The article was received on 10 May 2018, accepted on 16 May 2018 and first published on 16 May 2018


Article type: Communication
DOI: 10.1039/C8CC03769H
Citation: Chem. Commun., 2018, Advance Article
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    Label-free and high-throughput bioluminescence detection of uracil-DNA glycosylase in cancer cells through tricyclic cascade signal amplification

    Y. Zhang, Q. Li, C. Li and C. Zhang, Chem. Commun., 2018, Advance Article , DOI: 10.1039/C8CC03769H

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