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Quantification of methylation efficiency at a specific N6-methyladenosine position in rRNA by using BNA probes

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Abstract

We found that insertion of artificial nucleic acid analogs, such as bridged nucleic acid (BNA), into DNA probes increases the difference in melting temperature between N6-methyladenosine (m6A)-containing RNA and unmethylated RNA. By applying this principle, we quantified methylation efficiency at m6A sites in E. coli 23S rRNA with high accuracy.

Graphical abstract: Quantification of methylation efficiency at a specific N6-methyladenosine position in rRNA by using BNA probes

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Publication details

The article was received on 08 May 2018, accepted on 01 Aug 2018 and first published on 02 Aug 2018


Article type: Communication
DOI: 10.1039/C8CC03713B
Citation: Chem. Commun., 2018, Advance Article
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    Quantification of methylation efficiency at a specific N6-methyladenosine position in rRNA by using BNA probes

    T. Oshima, K. Ishiguro, T. Suzuki and Y. Kawahara, Chem. Commun., 2018, Advance Article , DOI: 10.1039/C8CC03713B

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