Reconstitution of full-length P450BM3 with an artificial metal complex by utilising the transpeptidase Sortase A

Keita Omura; Yuichiro Aiba; Hiroki Onoda; Joshua Kyle Stanfield; Shinya Ariyasu; Hiroshi Sugimoto; Yoshitsugu Shiro; Osami Shoji; Yoshihito Watanabe

doi:10.1039/C8CC02760A

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Reconstitution of full-length P450BM3 with an artificial metal complex by utilising the transpeptidase Sortase A†

Keita Omura,

^a Yuichiro Aiba,

^a Hiroki Onoda,

^a Joshua Kyle Stanfield,

^a Shinya Ariyasu,

^a Hiroshi Sugimoto,

^bc Yoshitsugu Shiro,

^d Osami Shoji

*^ab and Yoshihito Watanabe

*^e

Author affiliations

* Corresponding authors

^a Department of Chemistry, Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-0802, Japan
E-mail: shoji.osami@a.mbox.nagoya-u.ac.jp

^b Core Research for Evolutional Science and Technology (CREST), Japan Science and Technology Agency, 5 Sanban-cho, Chiyoda-ku, Tokyo, Japan

^c RIKEN SPring-8 Center, 1-1-1 Kouto, Sayo-cho, Hyogo, Japan

^d Department of Life Science, Graduate School of Life Science, University of Hyogo, 3-2-1 Kouto, Kamighori, Akoh, Hyogo, Japan

^e Research Center for Materials Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-0802, Japan

Abstract

Haem substitution is an effective approach to tweak the function of haemoproteins. Herein, we report a facile haem substitution method for self-sufficient cytochrome P450BM3 (CYP102A1) from Bacillus megaterium utilising the transpeptidase Sortase A from Staphylococcus aureus. We successfully constructed Mn-substituted BM3 and investigated its catalytic activity.

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Article information

DOI: https://doi.org/10.1039/C8CC02760A
Article type: Communication
Submitted: 06 Apr 2018
Accepted: 15 May 2018
First published: 22 May 2018

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Chem. Commun., 2018,54, 7892-7895

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Reconstitution of full-length P450BM3 with an artificial metal complex by utilising the transpeptidase Sortase A

K. Omura, Y. Aiba, H. Onoda, J. K. Stanfield, S. Ariyasu, H. Sugimoto, Y. Shiro, O. Shoji and Y. Watanabe, Chem. Commun., 2018, 54, 7892 DOI: 10.1039/C8CC02760A

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Chemical Communications

Reconstitution of full-length P450BM3 with an artificial metal complex by utilising the transpeptidase Sortase A†

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Reconstitution of full-length P450BM3 with an artificial metal complex by utilising the transpeptidase Sortase A

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