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Issue 49, 2018
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XNA ligation using T4 DNA ligase in crowding conditions

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Abstract

T4 DNA ligase is capable of ligating 2′OMe-RNA duplexes, HNA, LNA and FANA mixed sequences in the presence of 10% w/v PEG8000 and 3 M betaine. The enzymatic joining of oligonucleotides containing multiple consecutive XNA nucleotides at the ligation site has not been reported before.

Graphical abstract: XNA ligation using T4 DNA ligase in crowding conditions

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Publication details

The article was received on 26 Mar 2018, accepted on 30 May 2018 and first published on 31 May 2018


Article type: Communication
DOI: 10.1039/C8CC02414F
Citation: Chem. Commun., 2018,54, 6408-6411
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    XNA ligation using T4 DNA ligase in crowding conditions

    D. Kestemont, M. Renders, P. Leonczak, M. Abramov, G. Schepers, V. B. Pinheiro, J. Rozenski and P. Herdewijn, Chem. Commun., 2018, 54, 6408
    DOI: 10.1039/C8CC02414F

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