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Endogenous exosome labelling with an amphiphilic NIR-fluorescent probe

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Abstract

The recognition of the biological, diagnostic and medical importance of exosomes has given rise to an urgent need for efficient labelling of these extracellular vesicles in ways that do not alter their inherent characteristics. We report for the first time an endogenous method to NIR-fluorescent labelled exosomes using an amphiphilic probe without the need for immunolabelling or synthetic or chromatographic manipulation of exosomes. Comparative analyses of labelled and unlabelled exosomes with NTA, AFM, flow cytometry and immunoblot analysis all show a high degree of similarity. Spectroscopic analysis and fluorescence imaging confirmed the ability to visualise purified NIR-exosomes.

Graphical abstract: Endogenous exosome labelling with an amphiphilic NIR-fluorescent probe

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Publication details

The article was received on 16 Mar 2018, accepted on 27 May 2018 and first published on 14 Jun 2018


Article type: Communication
DOI: 10.1039/C8CC02135J
Citation: Chem. Commun., 2018, Advance Article
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    Endogenous exosome labelling with an amphiphilic NIR-fluorescent probe

    M. P. Monopoli, A. Zendrini, D. Wu, S. Cheung, G. Sampedro, B. Ffrench, J. Nolan, O. Piskareva, R. L. Stalings, S. Ducoli, P. Bergese and D. F. O’Shea, Chem. Commun., 2018, Advance Article , DOI: 10.1039/C8CC02135J

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