Gene assembly via one-pot chemical ligation of DNA promoted by DNA nanostructures†
Ilenia
Manuguerra, 
‡a
Stefano
Croce,
‡bc
Afaf H.
El-Sagheer,
de
Tom
Brown,
d
Kurt V.
Gothelf
a
and
Antonio
Manetto
*f
‡a
Stefano
Croce,
‡bc
Afaf H.
El-Sagheer,
de
Tom
Brown,
d
Kurt V.
Gothelf
a
and
Antonio
Manetto
*f
Abstract
Current gene synthesis methods are driven by enzymatic reactions. Here we report the one-pot synthesis of a chemically-ligated gene from 14 oligonucleotides. The chemical ligation benefits from the highly efficient click chemistry approach templated by DNA nanostructures, and produces modified DNA that is compatible with polymerase enzymes.
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