Issue 21, 2018

Electrochemical aptasensors for zeatin detection based on MoS2 nanosheets and enzymatic signal amplification

Abstract

A simple and sensitive electrochemical aptasensor was constructed for zeatin detection, where MoS2 nanosheets were used as the immobilization matrix for gold nanoparticles (AuNPs), and AuNPs were employed as the immobilization matrix to probe DNA. After the aptamer DNA and assist DNA hybridized with probe DNA, Y-type DNA can be formed with two biotins at the terminals of aptamer DNA. Then, avidin modified alkaline phosphatase (Avidin-ALP) can be further modified on the electrode surface through the biotin and avidin interaction. Under the catalytic effect of ALP, p-nitrophenylphosphate disodium (PNPP) can be hydrolyzed to produce p-nitrophenol (PNP). However, in the presence of zeatin, the formed Y-type DNA can be destroyed due to the formation of the zeatin–aptamer conjugate, which further reduces the amount of PNP and leads to the decrease of the oxidation signal of PNP. Under the optimum conditions, the change of the oxidation peak current of PNP was inversely proportional to the logarithm value of zeatin concentration in the range of 50 pM–50 nM. The detection limit was calculated to be 16.6 pM. This electrochemical method also showed good detection selectivity and stability. The potential applicability of this method was proved by detecting zeatin in real samples.

Graphical abstract: Electrochemical aptasensors for zeatin detection based on MoS2 nanosheets and enzymatic signal amplification

Supplementary files

Article information

Article type
Paper
Submitted
20 Jul 2018
Accepted
06 Sep 2018
First published
07 Sep 2018

Analyst, 2018,143, 5185-5190

Electrochemical aptasensors for zeatin detection based on MoS2 nanosheets and enzymatic signal amplification

Y. Zhou, H. Yin, Y. Wang, C. Sui, M. Wang and S. Ai, Analyst, 2018, 143, 5185 DOI: 10.1039/C8AN01356J

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