Issue 21, 2018

Ratiometric red-emission fluorescence detection of Al3+ in pure aqueous solution and live cells by a fluorescent peptidyl probe using aggregation-induced emission

Abstract

The development of a fluorescence method for the selective ratiometric detection of Al3+ ions in pure aqueous solutions and live cells is still a significant challenge. In the present study, we synthesized a new type of fluorescent probe using an Al3+-triggered self-assembly based on the dipeptide receptor and an aggregation-induced emission fluorophore. The fluorescent probe (1) bearing cyanostilbene with excitation by visible light detected Al3+ ions sensitively in pure aqueous buffered solution by ratiometric red-emission at 600 nm. 1 provided a highly selective ratiometric detection of Al3+ among 16 metal ions in aqueous solution. 1 exhibited sensitive ratiometric response to Al3+ in aqueous buffered solutions at pH ranging from 5 to 7.4. The detection limit (145 nM, R2 = 0.999) for Al3+ ions in pure aqueous solution was much lower than the maximum allowable level of Al3+ in drinking water demanded by the Environmental Protection Agency (EPA). The probe provided an efficient approach to detect low concentrations of Al3+ in ground water, tap water, and live cells by ratiometric red-emissions at 600 nm. The binding study using dynamic light scattering, NMR, IR, and TEM revealed that the complex between 1 and Al3+ self-assembled to form nanoparticles, resulting in the enhancement of the emission at 600 nm and a concomitant decrease in the emission at 535 nm.

Graphical abstract: Ratiometric red-emission fluorescence detection of Al3+ in pure aqueous solution and live cells by a fluorescent peptidyl probe using aggregation-induced emission

Supplementary files

Article information

Article type
Paper
Submitted
04 Jul 2018
Accepted
04 Sep 2018
First published
04 Sep 2018

Analyst, 2018,143, 5285-5294

Ratiometric red-emission fluorescence detection of Al3+ in pure aqueous solution and live cells by a fluorescent peptidyl probe using aggregation-induced emission

L. N. Neupane, P. K. Mehta, S. Oh, S. Park and K. Lee, Analyst, 2018, 143, 5285 DOI: 10.1039/C8AN01221K

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