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Analysis of time-course drug response in rat cardiomyocytes cultured on a pattern of islands

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Abstract

We previously reported the kinetics analysis of cardiomyocyte beating using scanning electrochemical microscopy (SECM). In this study, a stage-top incubator and a capillary micropipette (MP) for delivering drugs were assembled with an SECM instrument, and the responses of rat cardiomyocytes were analyzed under a culture environment after drug stimulation. When adenosine triphosphate (ATP) was delivered to synchronously beating cardiomyocytes, the beating acceleration effect of ATP was counteracted by the synchronously beating network in the culture dish. In contrast, cardiomyocytes cultured on a pattern of islands in a culture dish showed fluctuations in the duration of beating upon the addition of ATP. We also examined the effect of the cardiotoxic agent astemizole on cardiomyocytes and successfully detected motion fluctuations. Therefore, drug stimulation via MPs and beating measurement by SECM are effective routes for the evaluation of drug candidates through the analysis of time-course beating motion fluctuations of the cardiomyocytes.

Graphical abstract: Analysis of time-course drug response in rat cardiomyocytes cultured on a pattern of islands

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Publication details

The article was received on 05 Jun 2018, accepted on 10 Jul 2018 and first published on 20 Jul 2018


Article type: Paper
DOI: 10.1039/C8AN01033A
Citation: Analyst, 2018, Advance Article
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    Analysis of time-course drug response in rat cardiomyocytes cultured on a pattern of islands

    S. Okumura, Y. Hirano, Y. Maki and Y. Komatsu, Analyst, 2018, Advance Article , DOI: 10.1039/C8AN01033A

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