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Au nanocluster-embedded chitosan nanocapsules as labels for the ultrasensitive fluorescence immunoassay of Escherichia coli O157:H7

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Abstract

Herein, Au nanocluster (AuNC)-embedded chitosan (CS) nanocapsules were prepared and used as a novel signal amplification system for the sensitive fluorescence immunoassay of Escherichia coli O157:H7 (E. coli O157:H7). AuNCs were embedded in CS to form AuNCs@CS nanocapsules, where a large number of AuNCs were embedded in each nanocapsule. A rapid, ultrasensitive, and selective method for the fluorescence detection of E. coli O157:H7 was developed by coupling the nanocapsule-amplification strategy with immunological recognition and magnetic separation. Compared with a conventional immunoassay using AuNCs as labels, the fluorescence signals of the proposed immunoassay were greatly amplified, because much more fluorescent AuNCs are linked to each bacterial cell. As a result, a linear range was obtained from 3 to 700 CFU mL−1, with a detection limit of 1 CFU mL−1. This method was successfully used to detect E. coli O157:H7 in drinking water and milk samples.

Graphical abstract: Au nanocluster-embedded chitosan nanocapsules as labels for the ultrasensitive fluorescence immunoassay of Escherichia coli O157:H7

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Publication details

The article was received on 28 May 2018, accepted on 20 Jul 2018 and first published on 31 Jul 2018


Article type: Paper
DOI: 10.1039/C8AN00987B
Citation: Analyst, 2018, Advance Article
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    Au nanocluster-embedded chitosan nanocapsules as labels for the ultrasensitive fluorescence immunoassay of Escherichia coli O157:H7

    C. Cheng, L. Yang, M. Zhong, W. Deng, Y. Tan, Q. Xie and S. Yao, Analyst, 2018, Advance Article , DOI: 10.1039/C8AN00987B

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