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SEM-Raman image cytometry of cells

Abstract

A correlative and integrated Scanning Electron Microscopy (SEM) and Raman micro-spectroscopy is presented that enables the characterization and identification of different cancer and non-cancer cells through SEM-Raman image cytometry. The hybrid microscope system enables the acquisition of high resolution SEM images of un-coated cells and the spatial correlation with chemical information as obtained from Raman micro-spectroscopic imaging. A sample preparation protocol and workflow is presented that is compatible with the demands of hybrid SEM-Raman microscopy. Use has been made of stainless steel cell substrates that are both conductive and give low optical response in Raman scattering. Correlative and integrated SEM-Raman micro-spectroscopy is illustrated with cells from blood and cells from a SKBR-3 breast cancer cell line.

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Publication details

The article was received on 23 May 2018, accepted on 30 Jul 2018 and first published on 02 Aug 2018


Article type: Paper
DOI: 10.1039/C8AN00955D
Citation: Analyst, 2018, Accepted Manuscript
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    SEM-Raman image cytometry of cells

    A. Enciso-Martinez, F. Timmermans, A. Nanou, L. Terstappen and C. Otto, Analyst, 2018, Accepted Manuscript , DOI: 10.1039/C8AN00955D

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