Jump to main content
Jump to site search


Gold-nanostar-based SERS substrates for studying protein aggregation processes

Author affiliations

Abstract

Aggregation of proteins has been related to some neurodegenerative diseases such as Alzheimer's and Parkinson's among others. Raman spectroscopy is a useful technique for the investigation of protein conformation and of changes in their secondary structure. In this study, a surface enhanced Raman spectroscopy (SERS) substrate based on the immobilization of plasmonic gold nanostars on a glass slide via silanization of the surface has been prepared and characterized. Gold nanostars were synthesized via a seed-growth method using gold nanoparticles as seeds obtained via stainless steel as the reducing agent. The plasmonic substrate provided an enhancement of 5.7 × 102 fold, as shown for the Raman signal of crystal violet. Using this SERS-active substrate, the investigation of aggregation processes of bovine serum albumin (BSA) and myoglobin proteins upon temperature and solvent modification has been enabled with enhanced sensitivity. Both curve fitting and deconvolution of the amide I band, as well as 2D correlation analysis, were employed for the evaluation of the changes in the SERS spectra of the protein samples. The amide I band within the SERS spectra of the BSA protein revealed a decrease in the α-helix structures within the secondary structure of the protein while the presence of β-sheet structures increased with temperature and solvent concentration.

Graphical abstract: Gold-nanostar-based SERS substrates for studying protein aggregation processes

Back to tab navigation

Supplementary files

Publication details

The article was received on 30 Apr 2018, accepted on 13 Aug 2018 and first published on 14 Aug 2018


Article type: Paper
DOI: 10.1039/C8AN00804C
Citation: Analyst, 2018, Advance Article
  •   Request permissions

    Gold-nanostar-based SERS substrates for studying protein aggregation processes

    N. Schwenk, B. Mizaikoff, S. Cárdenas and Á. I. López-Lorente, Analyst, 2018, Advance Article , DOI: 10.1039/C8AN00804C

Search articles by author

Spotlight

Advertisements