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A reaction-based near-infrared fluorescent probe that can visualize endogenous selenocysteine in vivo in tumor-bearing mice

Abstract

Monitoring the fluctuations of endogenous selenocysteine (Sec) in vivo is of significant interest to understand the physiological roles of Sec and the mechanisms of Sec-relevant diseases. Herein, a new near-infrared fluorescence probe, Fsec-1, has been developed for the determination of endogenous Sec in living cells and in vivo. Fsec-1 exhibits large fluorescence enhancement (136-fold) and a remarkably large Stokes shift (195 nm) when reacted with Sec. With advantages of high sensitivity (a detection limit of 10 nM), good selectivity and low cytotoxicity, Fsec-1 was able to recognize both exogenous and endogenous Sec in living cells. The probe was also successfully applied in visualizing both exogenous and endogenous Sec in living mice. Notably, endogenously generated Sec in living tumors xenografted in nude mice were selectively detected by our reaction-based NIR probe for the first time. These results indicated that our new probe could serve as an efficient tool in monitoring endogenous Sec in vivo and exploring the anticancer mechanism of selenium.

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Supplementary files

Publication details

The article was received on 25 Apr 2018, accepted on 18 Jul 2018 and first published on 30 Jul 2018


Article type: Paper
DOI: 10.1039/C8AN00765A
Citation: Analyst, 2018, Accepted Manuscript
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    A reaction-based near-infrared fluorescent probe that can visualize endogenous selenocysteine in vivo in tumor-bearing mice

    L. Zhang, X. Kai, Y. Zhang, Y. Zheng, Y. Xue, X. Yin and J. Zhao, Analyst, 2018, Accepted Manuscript , DOI: 10.1039/C8AN00765A

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