Jump to main content
Jump to site search


Aptamer immobilization on amino-functionalized metal–organic frameworks: an ultrasensitive platform for the electrochemical diagnostic of Escherichia coli O157:H7

Author affiliations

Abstract

Herein, we report the development of an electrochemical biosensor for Escherichia coli O157:H7 diagnostic based on amino-functionalized metal–organic frameworks (MOFs) as a new generation of organic–inorganic hybrid nanocomposites. The electrical and morphological properties of MOFs were enhanced by interweaving each isolated MOF crystal with polyaniline (PANI). Subsequent attachment of the amine-modified aptamer to the polyanilinated MOFs was accomplished using glutaraldehyde (GA) as a cross-linking agent. The prepared biocompatible platform was carefully characterized by means of field-emission scanning electron microscopy (FESEM), energy-dispersive spectroscopy (EDS), Fourier transform infrared spectroscopy (FT-IR), and X-ray powder diffraction (XRD) techniques. The biosensor fabrication and its electrochemical characterizations were monitored by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) techniques. Differential pulse voltammetry (DPV) was applied to monitoring and quantitation of the interaction between the aptamer and E. coli O157:H7 using methylene blue (MB) as an electrochemical indicator. Changes in the reduction peak current of MB in the presence of E. coli O157:H7 was recorded as an analytical signal and indicated a relationship with the logarithm of the E. coli O157:H7 concentration in the range of 2.1 × 101 to 2.1 × 107 CFU mL−1 with a LOQ of 21 CFU mL−1 and LOD of 2 CFU mL−1. The electrochemical aptasensor displayed good recovery values for the detection of E. coli O157:H7 in environmental real samples and also could act as a smart device to investigate the effects of antibacterial agents against E. coli O157:H7.

Graphical abstract: Aptamer immobilization on amino-functionalized metal–organic frameworks: an ultrasensitive platform for the electrochemical diagnostic of Escherichia coli O157:H7

Back to tab navigation

Supplementary files

Publication details

The article was received on 19 Apr 2018, accepted on 05 Jun 2018 and first published on 05 Jun 2018


Article type: Paper
DOI: 10.1039/C8AN00725J
Citation: Analyst, 2018, Advance Article
  •   Request permissions

    Aptamer immobilization on amino-functionalized metal–organic frameworks: an ultrasensitive platform for the electrochemical diagnostic of Escherichia coli O157:H7

    S. Shahrokhian and S. Ranjbar, Analyst, 2018, Advance Article , DOI: 10.1039/C8AN00725J

Search articles by author

Spotlight

Advertisements