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Dual-signal ratiometric electrochemiluminescence assay for detecting the activity of human methyltransferase

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Abstract

In this paper, a dual-signal ratiometric electrochemiluminescence (ECL) assay was developed to detect human DNA (cytosine-5)-methyltransferase1 (DNMT1) activity. Mercaptopropionic acid (MPA)-modified Eu3+-doped CdS nanocrystals (MPA-CdS:Eu NCs) and luminol were used as two different ECL emitters, which showed ECL signals at different electrochemical potentials. The resultant ECL peaks exhibited opposite trends in the DNMT1 system. According to the ratio of the ECL responses of CdS to luminol, the DNMT1 activity was detected in the range of 1.0–30.0 U mL−1 in buffer solution. The limit of detection (LOD) was estimated to be 0.07 U mL−1 at a signal-to-noise ratio of 3. The assay was extended to detect the DNMT1 activity from crude lysates of cancer cells, along with the effect of DNMT1 inhibitors such as 5-aza and 5-aza-2′-dC.

Graphical abstract: Dual-signal ratiometric electrochemiluminescence assay for detecting the activity of human methyltransferase

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Publication details

The article was received on 02 Apr 2018, accepted on 07 Jun 2018 and first published on 08 Jun 2018


Article type: Paper
DOI: 10.1039/C8AN00611C
Citation: Analyst, 2018, Advance Article
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    Dual-signal ratiometric electrochemiluminescence assay for detecting the activity of human methyltransferase

    Z. Guo, B. Qiao, Q. Guo, H. Zhang, C. Cai and J. Feng, Analyst, 2018, Advance Article , DOI: 10.1039/C8AN00611C

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