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A novel porphyrin-based near-infrared fluorescent probe for hypochlorite detection and its application in vitro and in vivo

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Abstract

Reactive oxygen species (ROS), especially HOCl/ClO, have been demonstrated to play essential roles in both physiological and pathological processes, and an abnormal level of HOCl/ClO is related to some diseases. In this work, a very fast responsive (within 30 seconds) porphyrin-based fluorescent probe, TPP-TCF, for ClO with a NIR emissive wavelength was prepared. This probe exhibited excellent selectivity towards ClO and would not be interfered with by other ROS and typical nucleophiles. The limit of detection (LOD) for ClO was evaluated to be 0.29 μM, indicating high sensitivity towards ClO. In further bioimaging experiments, TPP-TCF displayed low-cytotoxicity and good cell penetrability for recognizing exogenous ClO in HeLa cells. Moreover, this probe was successfully applied in imaging endogenous ClO in living animals.

Graphical abstract: A novel porphyrin-based near-infrared fluorescent probe for hypochlorite detection and its application in vitro and in vivo

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Publication details

The article was received on 28 Mar 2018, accepted on 18 Apr 2018 and first published on 19 Apr 2018


Article type: Paper
DOI: 10.1039/C8AN00586A
Citation: Analyst, 2018, Advance Article
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    A novel porphyrin-based near-infrared fluorescent probe for hypochlorite detection and its application in vitro and in vivo

    X. Wang, J. Min, W. Wang, Y. Wang, G. Yin and R. Wang, Analyst, 2018, Advance Article , DOI: 10.1039/C8AN00586A

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