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Issue 16, 2018
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Sequence-specific 5mC detection in live cells based on the TALE-split luciferase complementation system

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Abstract

We established a method for converting TALE–DNA binding to luminescence, by combining a TALE and a split luciferase system. Furthermore, using a methylation-sensitive TALE, sequence-specific 5mC detection of genomic DNA was achieved in live cells. This study provides a new strategy for exploring the biological functions of 5mC.

Graphical abstract: Sequence-specific 5mC detection in live cells based on the TALE-split luciferase complementation system

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Publication details

The article was received on 25 Mar 2018, accepted on 11 Jul 2018 and first published on 17 Jul 2018


Article type: Communication
DOI: 10.1039/C8AN00562A
Citation: Analyst, 2018,143, 3793-3797
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    Sequence-specific 5mC detection in live cells based on the TALE-split luciferase complementation system

    S. Tsuji, K. Shinoda, S. Futaki and M. Imanishi, Analyst, 2018, 143, 3793
    DOI: 10.1039/C8AN00562A

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