Sequence-specific 5mC detection in live cells based on the TALE-split luciferase complementation system

Shogo Tsuji; Kouki Shinoda; Shiroh Futaki; Miki Imanishi

doi:10.1039/C8AN00562A

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Sequence-specific 5mC detection in live cells based on the TALE-split luciferase complementation system†

Shogo Tsuji,^a Kouki Shinoda,^a Shiroh Futaki

^a and Miki Imanishi

*^a

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* Corresponding authors

^a Institute for Chemical Research, Kyoto University, Uji, Kyoto 611-0011, Japan
E-mail: imiki@scl.kyoto-u.ac.jp

Abstract

We established a method for converting TALE–DNA binding to luminescence, by combining a TALE and a split luciferase system. Furthermore, using a methylation-sensitive TALE, sequence-specific 5mC detection of genomic DNA was achieved in live cells. This study provides a new strategy for exploring the biological functions of 5mC.

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Article information

DOI: https://doi.org/10.1039/C8AN00562A
Article type: Communication
Submitted: 25 Mar 2018
Accepted: 11 Jul 2018
First published: 17 Jul 2018

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Analyst, 2018,143, 3793-3797

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Sequence-specific 5mC detection in live cells based on the TALE-split luciferase complementation system

S. Tsuji, K. Shinoda, S. Futaki and M. Imanishi, Analyst, 2018, 143, 3793 DOI: 10.1039/C8AN00562A

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Sequence-specific 5mC detection in live cells based on the TALE-split luciferase complementation system†

Abstract

Supplementary files

Article information

Download Citation

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Sequence-specific 5mC detection in live cells based on the TALE-split luciferase complementation system

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