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A coumarin-based fluorescent probe for monitoring labile ferrous iron in living systems

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Abstract

Labile Fe2+ has been considered to be a metabolically active and regulatory form of cellular iron. Monitoring the dynamic level of labile Fe2+ in biological systems is vital for evaluating the iron related biological processes and diseases as well as dissecting the exact physiological and pathophysiological functions of the labile Fe2+. Herein, we rationally constructed a coumarin-based fluorescent probe for sensing labile Fe2+ in living systems based on a novel Fe2+ meditated cyclization reaction strategy. The probe showed a highly selective and sensitive response to Fe2+, and the detection limit was determined to be 45 nM. Significantly, the probe displayed fast response to Fe2+, with the sensing reaction completed in 2 min, which is beneficial for real time sensing. The application of the probe for sensing different concentrations of labile Fe2+ in living cells has been conducted. In addition, the basal and endogenous levels of labile Fe2+ in living systems were also successfully monitored.

Graphical abstract: A coumarin-based fluorescent probe for monitoring labile ferrous iron in living systems

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Publication details

The article was received on 24 Mar 2018, accepted on 24 Apr 2018 and first published on 24 Apr 2018


Article type: Paper
DOI: 10.1039/C8AN00556G
Citation: Analyst, 2018, Advance Article
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    A coumarin-based fluorescent probe for monitoring labile ferrous iron in living systems

    L. Long, N. Wang, Y. Han, M. Huang, X. Yuan, S. Cao, A. Gong and K. Wang, Analyst, 2018, Advance Article , DOI: 10.1039/C8AN00556G

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