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WS2 nanosheets sensitized CdS quantum dots heterostructure for photoelectrochemical immunoassay of alpha-fetoprotein coupled with enzyme-mediated biocatalytic precipitation

Abstract

Based on enzymatic biocatalytic precipitation to quench the amplified photocurrent response that generated from WS2 nanosheets sensitized CdS quantum dots heterostructure, a novel photoelectrochemical immunosensor was constructed for sensitive alpha-fetoprotein (AFP) monitoring, in which anti-AFP antibody and horseradish peroxidase-tagged anti-AFP antibody (HRP-Ab2) served as capture unit and signal indicator, respectively. In the presence of AFP, HRP-Ab2 was immobilized on electrode surface for establishing sandwich-type immunocomplex via antigen-antibody interaction, and subsequently catalyzed 4-chloro-1-naphthol to form the nonconductive and insoluble precipitation, which effectively impeded the diffusion and electron transfer of electron donor, achieving the decreased photocurrent. Under optimized conditions, the excellent linearity range (1 pg mL-1 − 20 ng mL-1), satisfactory detection limit (0.43 pg mL-1), desirable selectivity and good stability were obtained for designed immunosensor. Moreover, the application of AFP determination in human serum allowed it to hold great potential for early diagnostics of some cancer diseases.

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Publication details

The article was received on 23 Mar 2018, accepted on 13 May 2018 and first published on 14 May 2018


Article type: Paper
DOI: 10.1039/C8AN00551F
Citation: Analyst, 2018, Accepted Manuscript
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    WS2 nanosheets sensitized CdS quantum dots heterostructure for photoelectrochemical immunoassay of alpha-fetoprotein coupled with enzyme-mediated biocatalytic precipitation

    Y. Zang, Y. Ju, X. Hu, H. Zhou, Z. Yang, J. Jiang and H. Xue, Analyst, 2018, Accepted Manuscript , DOI: 10.1039/C8AN00551F

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