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A highly sensitive SPRi biosensing strategy for simultaneous detection of multiplex miRNAs based on strand displacement amplification and AuNPs signal enhancement

Abstract

Herein, a dual channel surface plasmon resonance imaging (SPRi) biosensor has been developed for simultaneous and highly sensitive detection of multiplex miRNAs based on strand displacement amplification (SDA) and DNA-functionalized AuNPs signal enhancement. In the presence of target miRNAs (miR-21 or miR-192), the miRNAs could specifically hybridize with the corresponding hairpin probes (H) and initiate the SDA, resulting in massive triggers. Subsequently, the two parts of the released triggers could hybridize with capture probes (CP) and DNA-functionalized AuNPs, assembling DNA sandwiches with great mass on the chip surface. A significantly amplified SPR signal readout was achieved. The established biosensing method was capable of simultaneously detecting multiplex miRNAs with a limit of detection down to 0.15 pM for miR-21 and 0.22 pM for miR-192, respectively. The method exhibited good specificity and acceptable reproducibility. Moreover, the developed method was applied to the determination of target miRNAs in complex matrix. Thus, this developed SPRi biosensing method might present a potential alternative tool for miRNAs detection in biomedical research and clinical diagnosis.

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Publication details

The article was received on 23 Mar 2018, accepted on 14 May 2018 and first published on 16 May 2018


Article type: Paper
DOI: 10.1039/C8AN00549D
Citation: Analyst, 2018, Accepted Manuscript
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    A highly sensitive SPRi biosensing strategy for simultaneous detection of multiplex miRNAs based on strand displacement amplification and AuNPs signal enhancement

    X. Wei, X. Duan, X. Zhou, J. Wu, H. Xu, X. Min and S. Ding, Analyst, 2018, Accepted Manuscript , DOI: 10.1039/C8AN00549D

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