Jump to main content
Jump to site search

Issue 15, 2018
Previous Article Next Article

One-step isothermal detection of multiple KRAS mutations by forming SNP specific hairpins on a gold nanoshell

Author affiliations

Abstract

We developed a one-step isothermal method for typing multiple KRAS mutations using a designed set of primers to form a hairpin on a gold nanoshell upon being ligated by a SNP specific DNA ligase after binding of targets. As a result, we could detect as low as 20 attomoles of KRAS mutations within 1 h.

Graphical abstract: One-step isothermal detection of multiple KRAS mutations by forming SNP specific hairpins on a gold nanoshell

Back to tab navigation

Supplementary files

Publication details

The article was received on 21 Mar 2018, accepted on 09 Apr 2018 and first published on 11 Apr 2018


Article type: Communication
DOI: 10.1039/C8AN00525G
Citation: Analyst, 2018,143, 3544-3548
  •   Request permissions

    One-step isothermal detection of multiple KRAS mutations by forming SNP specific hairpins on a gold nanoshell

    C. H. Chung and J. H. Kim, Analyst, 2018, 143, 3544
    DOI: 10.1039/C8AN00525G

Search articles by author

Spotlight

Advertisements