Issue 15, 2018
From the journal:

Analyst

One-step isothermal detection of multiple KRAS mutations by forming SNP specific hairpins on a gold nanoshell†

Chan Ho Chung ORCID logo a  and  Joong Hyun Kim ORCID logo *a  

* Corresponding authors

a Medical Device Development Center, Daegu-Gyeongbuk Medical Innovation Foundation, Daegu, Republic of Korea
E-mail: jhkim@dgmif.re.kr

Abstract

We developed a one-step isothermal method for typing multiple KRAS mutations using a designed set of primers to form a hairpin on a gold nanoshell upon being ligated by a SNP specific DNA ligase after binding of targets. As a result, we could detect as low as 20 attomoles of KRAS mutations within 1 h.

Graphical abstract: One-step isothermal detection of multiple KRAS mutations by forming SNP specific hairpins on a gold nanoshell

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Article information

DOI
https://doi.org/10.1039/C8AN00525G
Article type
Communication
Submitted
21 Mar 2018
Accepted
09 Apr 2018
First published
11 Apr 2018

Analyst, 2018,143, 3544-3548

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One-step isothermal detection of multiple KRAS mutations by forming SNP specific hairpins on a gold nanoshell

C. H. Chung and J. H. Kim, Analyst, 2018, 143, 3544 DOI: 10.1039/C8AN00525G

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