Jump to main content
Jump to site search


Molecular profiling of single axons and dendrites in living neurons using electrosyringe-assisted electrospray mass spectrometry

Author affiliations

Abstract

The molecular profiling of single axons and dendrites in living neurons could provide important information for the better understanding of neuron function. Here, electrosyringe-assisted electrospray mass spectrometry (MS) is established for the first time to achieve intracellular sampling from one axon or dendrite in living neurons for mass spectrometric analysis. The key is the insertion of a ∼130 nm capillary tip into one axon or dendrite to load the cytosol through electro-osmotic flow. The ionization efficiency from the nano-capillary is enhanced to guarantee mass spectrometric analysis of multiple components from the axon and dendrite. Higher levels of pyroglutamic acid and glutamic acid are revealed in the axon compared to in the body and dendrite. This uneven distribution is in accordance with the accumulation of neurotransmitters in the axon for information delivery. The achievement of electrosyringe-assisted electrospray MS is to unveil the molecular distribution in the whole living neuron, which offers the feasibility to deeply investigate molecular communication between the axon/dendrite and the body inside neurons.

Graphical abstract: Molecular profiling of single axons and dendrites in living neurons using electrosyringe-assisted electrospray mass spectrometry

Back to tab navigation

Supplementary files

Publication details

The article was received on 15 Mar 2018, accepted on 30 Jul 2018 and first published on 30 Jul 2018


Article type: Paper
DOI: 10.1039/C8AN00483H
Citation: Analyst, 2018, Advance Article
  •   Request permissions

    Molecular profiling of single axons and dendrites in living neurons using electrosyringe-assisted electrospray mass spectrometry

    M. Xu, R. Pan, Y. Zhu, D. Jiang and H. Chen, Analyst, 2018, Advance Article , DOI: 10.1039/C8AN00483H

Search articles by author

Spotlight

Advertisements