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Miniature diamond-anvil cell for FTIR-microspectroscopy of small quantities of biosamples

Abstract

Fourier transform infrared (FTIR) spectroscopy techniques and data analyses have become widely available, easy to use, and convenient for studies of various biosamples, especially in biomedical science. Yet, cultivation of cells and purification of cell components is costly, often methodically challenging, and time and labor consuming. Therefore, reduction of sample amount is of high value. Here we propose a novel method for analysis of small quantities of biosamples by FTIR-microscopy of dry films using diamond-anvil cell (DAC). This approach allows to decrease the sample volume at least hundred times compared to a high-throughput screening device with zinc selenide microplates while still obtaining homogenous films, acquiring qualitative spectra, and using conventional 15x objective instead of ATR-objective. Both FTIR methods were applied for analyses of human colorectal cancer cell lines SW480 and SW620 cultured under hypoxic conditions to estimate the strengths and weaknesses of each approach. FTIR absorption spectra acquired by both methods were compared and no significant spectral differences were detected. It was shown that FTIR-microscopy of films on DAC can be used for evaluation, screening, discrimination and identification of biochemical markers in biosamples like cells. We conclude that the DAC can be transferred to other biosamples like tissues, biofluids, their components and extracellular matrix, and is especially valuable when the available quantities of biosamples is limited.

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Publication details

The article was received on 08 Mar 2018, accepted on 08 Jun 2018 and first published on 08 Jun 2018


Article type: Paper
DOI: 10.1039/C8AN00432C
Citation: Analyst, 2018, Accepted Manuscript
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    Miniature diamond-anvil cell for FTIR-microspectroscopy of small quantities of biosamples

    M. Grube, K. Shvirksts, C. Kraft, S. Kokorevicha, E. Zandberga, A. Abols, A. Line and U. Kalnenieks, Analyst, 2018, Accepted Manuscript , DOI: 10.1039/C8AN00432C

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