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Issue 10, 2018
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Identification and characterization of different tissues in blood vessel by multiplexed fluorescence lifetimes

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Abstract

Herein, fluorescence lifetime imaging microscopy (FLIM) was used to directly measure eosin fluorescence lifetimes from H&E-stained umbilical artery, and a further utilization of eosin for high-content and multi-target analysis was proposed for the first time. Smooth muscles, collagens, and elastic fibers can be distinguished by eosin fluorescence lifetimes (P < 0.001). Erythrocytes, smooth muscles, elastic fibers, and type I and III collagen from the H&E-stained umbilical artery can be simultaneously identified by multiplexed fluorescence lifetimes of eosin. Use of eosin and lifetime-based separation is a potential method to simplify the special staining for clinicopathologic examination. Multiplexed eosin fluorescence lifetimes may be a newly developed method that can directly determine the relative content of elastic fiber and collagens from the H&E-stained sections. FLIM may have potential applications as an assisted tool in the assessment of the severity and complexity of cardiovascular diseases.

Graphical abstract: Identification and characterization of different tissues in blood vessel by multiplexed fluorescence lifetimes

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Publication details

The article was received on 02 Mar 2018, accepted on 25 Mar 2018 and first published on 27 Mar 2018


Article type: Paper
DOI: 10.1039/C8AN00392K
Citation: Analyst, 2018,143, 2243-2248
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    Identification and characterization of different tissues in blood vessel by multiplexed fluorescence lifetimes

    T. Luo, D. Lin, T. Zhou, Y. Lu, S. Liu and J. Qu, Analyst, 2018, 143, 2243
    DOI: 10.1039/C8AN00392K

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