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Smart blood spots for whole blood protein analysis

Abstract

A reactor for whole blood sampling integrated with instant protein digestion in a “lab-on-paper” format is introduced here. The sampling reactor was fabricated on commercial available filter paper using HEMA-VDM polymerization followed by immobilization of trypsin. Immobilization conditions were investigated with respect to temperature, enzyme amount and immobilization time. The highest reactor efficiency with respect to protein digestion was obtained with 1.25 mg trypsin per reactor immobilized at room temperature for 3 hours. Four commercially available cellulose filter papers and DMPK-C cards were modified and immobilized with trypsin prior to whole blood sampling. Filter paper specifications including thickness (180 – 220 µm), weight (77 – 92 g/m2) and porosity (11 – 25 µm) where investigated with respect to performance (digestion efficiency and extraction recovery). From this study, a medium thickness paper with higher weight and porosity is optimal for reactor efficiency. The reactors were tested and compared with respect to a standard dried blood spot procedure for protein digestion. Peak performance was obtained at 134 ± 14 and 124 ± 7 high confidence protein groups for freeze thawed and fresh whole blood samples, respectively.

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Publication details

The article was received on 19 Feb 2018, accepted on 13 May 2018 and first published on 15 May 2018


Article type: Paper
DOI: 10.1039/C8AN00317C
Citation: Analyst, 2018, Accepted Manuscript
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    Smart blood spots for whole blood protein analysis

    Ø. Skjærvø, T. G. Halvorsen and L. Reubsaet, Analyst, 2018, Accepted Manuscript , DOI: 10.1039/C8AN00317C

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