Jump to main content
Jump to site search


Understanding the viral load during the synthesis and after rebinding of virus imprinted particles via real-time quantitative PCR

Author affiliations

Abstract

In the present study, virus imprinted particles have been synthesized for recognizing and specifically binding viruses. These materials may be used for biomimetic sensing schemes and for selective removal of virus particles. Virus imprinting procedures require careful optimization of the synthesis route for obtaining selective and efficiently binding imprinted materials. A remaining limitation has been a facile method for the quantification of the viral load during the imprinting process. Herein, human adenovirus (AdV) was selected as a model virus facilitating the development and application of a rapid virus quantification method based on a molecular biological approach. A real-time quantitative polymerase chain reaction, a.k.a., the qPCR method was developed for monitoring the AdV viral load during the synthesis of AdV imprinted particles, and subsequent rebinding studies. The developed analytical strategy allows the direct, rapid, and sensitive quantification of human adenovirus type 5 concentrations during synthesis and application of AdV imprinted polymers (AdV-MIPs) with a broad dynamic range suitable for both application scenarios. In addition, it was demonstrated by gel electrophoresis analysis that viruses indeed bind to the beads even after several washing steps.

Graphical abstract: Understanding the viral load during the synthesis and after rebinding of virus imprinted particles via real-time quantitative PCR

Back to tab navigation

Supplementary files

Publication details

The article was received on 15 Feb 2018, accepted on 12 Apr 2018 and first published on 13 Apr 2018


Article type: Paper
DOI: 10.1039/C8AN00300A
Citation: Analyst, 2018, Advance Article
  •   Request permissions

    Understanding the viral load during the synthesis and after rebinding of virus imprinted particles via real-time quantitative PCR

    M. Gast, S. Kühner, H. Sobek and B. Mizaikoff, Analyst, 2018, Advance Article , DOI: 10.1039/C8AN00300A

Search articles by author

Spotlight

Advertisements