Jump to main content
Jump to site search


A peptide-WS2 nanosheet based biosensing platform for determination of β-secretase and screening of its Inhibitors

Abstract

β-secretase (BACE1) is an important drug target in the treatment of Alzheimer’s disease (AD). Therefore, sensitive detection of BACE1 is essential for AD treatment and drug discovery. In this work, a facile and sensitive fluorescence biosensing platform was developed for BACE1 detection. This sensing platform was constituted based on the interaction between the WS2 nanosheet and peptide sequence. In the absence of BACE1, a FAM-labeled peptide substrate could be adsorbed on the surface of the WS2 nanosheet, thereby quenching its fluorescence. However, in the presence of BACE1, the hydrolysis of peptide substrate by BACE1 triggers could occur and subsequently released short FAM-linked peptide fragments which could not be adsorbed on the surface of WS2 nanosheet. This resulted in weak fluorescence quenching, thus, restoring the fluorescence signal. By measuring the change in fluorescence of the FAM-labeled peptide substrate, the fluorescent sensing platform based on WS2 nanosheet could monitor BACE1. The proposed WS2 nanosheet-based platform exhibited excellent specificity and high sensitivity with a detection limit of 66 pM for BACE1. Importantly, we also demonstrated that this platform was suitable for screening of BACE1 inhibitors. The proposed sensing platform not only provides a novel strategy for the BACE1 assay but also offers a potential tool for screening drugs.

Back to tab navigation

Supplementary files

Publication details

The article was received on 23 Jan 2018, accepted on 31 Jul 2018 and first published on 03 Aug 2018


Article type: Paper
DOI: 10.1039/C8AN00132D
Citation: Analyst, 2018, Accepted Manuscript
  •   Request permissions

    A peptide-WS2 nanosheet based biosensing platform for determination of β-secretase and screening of its Inhibitors

    X. Zuo, H. Dai, H. Zhang, J. Liu, S. Ma and X. Chen, Analyst, 2018, Accepted Manuscript , DOI: 10.1039/C8AN00132D

Search articles by author

Spotlight

Advertisements