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Estimating and correcting interference fringes in infrared spectra in infrared hyperspectral imaging

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Abstract

Short-term acclimation response of individual cells of Thalassiosira weissflogii was monitored by Synchrotron FTIR imaging over the span of 75 minutes. The cells, collected from batch cultures, were maintained in a constant flow of medium, at an irradiance of 120 μmol m−2 s−1 and at 20 °C. Multiple internal reflections due to the micro fluidic channel were modeled, and showed that fringes are additive sinusoids to the pure absorption of the other components of the system. Preprocessing of the hyperspectral cube (x, y, Abs(λ)) included removing spectral fringe using an EMSC approach. Principal component analysis of the time series of hyperspectral cubes showed macromolecular pool variations (carbohydrates, lipids and DNA/RNA) of less than 2% after fringe correction.

Graphical abstract: Estimating and correcting interference fringes in infrared spectra in infrared hyperspectral imaging

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Publication details

The article was received on 16 Jan 2018, accepted on 27 Jun 2018 and first published on 03 Sep 2018


Article type: Paper
DOI: 10.1039/C8AN00093J
Citation: Analyst, 2018, Advance Article
  • Open access: Creative Commons BY-NC license
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    Estimating and correcting interference fringes in infrared spectra in infrared hyperspectral imaging

    G. Azarfar, E. Aboualizadeh, N. M. Walter, S. Ratti, C. Olivieri, A. Norici, M. Nasse, A. Kohler, M. Giordano and C. J. Hirschmugl, Analyst, 2018, Advance Article , DOI: 10.1039/C8AN00093J

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