Jump to main content
Jump to site search

Issue 18, 2018
Previous Article Next Article

A comprehensive urinary steroid analysis strategy using two-dimensional gas chromatography – time of flight mass spectrometry

Author affiliations

Abstract

Steroids are key players in a high variety of physiological processes and are typically analyzed for the diagnosis of hormonal disorders. Due to their chemical and structural similarity many of these metabolites cannot be separated by conventional techniques such as liquid chromatography. Herein, we present an analysis strategy based on two dimensional gas chromatography (GC×GC) coupled to time-of-flight mass spectrometry (TOF MS) which demonstrates superior separation power and enables comprehensive screening of steroids. We show absolute quantitation of 40 steroids in human urine over three orders of magnitude with limits of detection ≤50 nM and the tentative identification of additional 30 steroids based on accurate mass, isotopic pattern analysis and spectral similarity matching to known steroids. The method displays excellent inter- and intra-day stability, repeatability and recovery and was validated for clinical routine analysis. Additionally, we demonstrate the potential of the approach for untargeted analysis of urinary steroids in mouse and rat.

Graphical abstract: A comprehensive urinary steroid analysis strategy using two-dimensional gas chromatography – time of flight mass spectrometry

Back to tab navigation

Supplementary files

Publication details

The article was received on 08 Dec 2017, accepted on 21 Aug 2018 and first published on 22 Aug 2018


Article type: Paper
DOI: 10.1039/C7AN01990D
Citation: Analyst, 2018,143, 4484-4494
  •   Request permissions

    A comprehensive urinary steroid analysis strategy using two-dimensional gas chromatography – time of flight mass spectrometry

    A. Bileck, S. N. Verouti, G. Escher, B. Vogt and M. Groessl, Analyst, 2018, 143, 4484
    DOI: 10.1039/C7AN01990D

Search articles by author

Spotlight

Advertisements