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Issue 88, 2017, Issue in Progress
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A novel fluorescence polarization assay for copper ions based on DNA-templated click chemistry and amplification of nanoparticles

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Abstract

In this work, we developed a novel fluorescence polarization (FP) method for the sensitive detection of Cu2+ based on the chemical ligation of DNA by click chemistry and silica nanoparticles-assisted FP enhancement. A double-stranded DNA hybrid (P1P2) is used as an “end-of-helix” template with an alkyne group and an azide group at the end of two DNA strands, respectively. Cu2+ can be reduced to Cu+ by sodium ascorbate, and trigger the chemical ligation P1P2 through the Cu+-catalyzed azide–alkyne cycloaddition. A biotin-labeled P3 which can hybridize with P1 is introduced to displace the FAM-contained P2 from P1P2 duplex. Since P1 and P2 are linked after the Cu2+-triggered click chemistry, a P1P2P3 complex is formed which can be captured on the silica nanoparticle surface through the biotin-streptavidin interaction, thus achieving a high FP signal. In the absence of Cu2+, there is no ligation between P1 and P2. The released P2 is free and cannot be immobilized on the nanoparticle surface, resulting in a low FP. The specificity of click chemistry provides a high selectivity, and the nanoparticle-assisted FP enhancement provides a high sensitivity with a detection limit of 0.0178 μM. This method also displays good assay performance in real water samples, which may be further used for environmental monitoring.

Graphical abstract: A novel fluorescence polarization assay for copper ions based on DNA-templated click chemistry and amplification of nanoparticles

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Publication details

The article was received on 10 Oct 2017, accepted on 04 Dec 2017 and first published on 08 Dec 2017


Article type: Paper
DOI: 10.1039/C7RA11159B
Citation: RSC Adv., 2017,7, 55668-55672
  • Open access: Creative Commons BY-NC license
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    A novel fluorescence polarization assay for copper ions based on DNA-templated click chemistry and amplification of nanoparticles

    S. Liao, J. Zhao, Y. Qin and S. Zhao, RSC Adv., 2017, 7, 55668
    DOI: 10.1039/C7RA11159B

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