Fast screening of whole blood samples for early detection and monitoring of thyroid diseases

Abstract

This paper described a fast and reliable method for the screening of whole blood for TSH, L-T₄, L-T₃ and D-T₄ using stochastic sensors. The main advantage of this method is the possibility of tracing the precise diagnosis of thyroid dysfunction from a single whole blood sample by multianalyte screening in one run, with low cost. Six stochastic microsensors based on mixtures between inulins: Frutafruit TEX (TEX) and inulin Inutec (IN), and ionic liquids: L-phenylalanine-tert-butyl-ester-lactate (L-PheC₄-Lac), L-alanine-tert-butyl-ester-L-lactate (L-AlaC₄-Lac) and L-alanine-tert-butyl-ester-nitrate (L-AlaC₄-NO₃), physically immobilized in a diamond paste matrix were designed and used for the screening tests. The tests were reliable for the assay of TSH, L-T₄, L-T₃ and D-T₄ in whole blood samples, with recoveries higher than 98.00% with RSD values lower than 1.00% being recorded.