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Issue 60, 2017, Issue in Progress
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Microvesicle release and micellar attack as the alternative mechanisms involved in the red-blood-cell-membrane solubilization induced by arginine-based surfactants

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Abstract

Two novel arginine-based surfactants, Bz-Arg-NHC10 and Bz-Arg-NHC12, were characterized with respect to surface properties and their interaction with human red-blood-cell (HRBC) membranes. The values for critical micellar concentration (CMC), the maximum surfactant adsorption at the air–liquid interface, and the area per molecule indicated better surface properties for Bz-Arg-NHC12. The observation of cylindrical worm-like aggregates of Bz-Arg-NHCn via atomic-force microscopy supported the predictions based on the value of the surfactant-packing parameter (SPP). Erythrocyte-membrane solubilization was effected by surfactant aggregates since cell lysis became evident at only surfactant concentrations above the CMC. Changes in HRBC shape observed at different surfactant concentrations led to the conclusion that a slow mechanism based on the insertion of surfactant monomers into the HRBC membrane, followed by a shedding of microvesicles was responsible for the hemolysis produced by both surfactants at the lower concentrations tested. In contrast, the extraction of membrane lipids upon collisions between HRBCs and surfactant aggregates competes with and prevents microvesicle release at the higher concentrations assayed.

Graphical abstract: Microvesicle release and micellar attack as the alternative mechanisms involved in the red-blood-cell-membrane solubilization induced by arginine-based surfactants

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Publication details

The article was received on 29 Mar 2017, accepted on 09 Jun 2017 and first published on 28 Jul 2017


Article type: Paper
DOI: 10.1039/C7RA03640J
Citation: RSC Adv., 2017,7, 37549-37558
  • Open access: Creative Commons BY license
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    Microvesicle release and micellar attack as the alternative mechanisms involved in the red-blood-cell-membrane solubilization induced by arginine-based surfactants

    M. E. Fait, M. Hermet, F. Comelles, P. Clapés, H. A. Alvarez, E. Prieto, V. Herlax, S. R. Morcelle and L. Bakás, RSC Adv., 2017, 7, 37549
    DOI: 10.1039/C7RA03640J

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