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Issue 24, 2017, Issue in Progress
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Dually sensitive dextran-based micelles for methotrexate delivery

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Abstract

Temperature-sensitive polymeric micelles were prepared from dextran grafted with poly(N-isopropylacrylamide) (PNIPAAm) or polyethylene glycol methyl ether (PEGMA) via controlled radical polymerization and evaluated as delivery systems of the anticancer drug methotrexate (MTX). Polymer-grafting was carried out after introduction of initiating groups onto the polysaccharide backbone, without the need for protection of hydroxyl groups and avoiding the use of toxic solvents. Temperature-responsive dextran-based copolymers were designed to exhibit self-aggregation behaviour, affinity for MTX and high cellular internalization. In addition, some grafted polymers incorporated 2-aminoethyl methacrylate to reinforce MTX encapsulation in the micelles by means of ionic interactions. Dextran-based micelles were cytocompatible and had an appropriate size to be used as drug carriers. MTX release was dependent on the pH and temperature. The combination of poly(2-aminoethylmethacrylate) and PNIPAAm with the dextran backbone permitted the complete release of MTX at normal physiological temperature. Co-polymer micelles were highly internalized by tumour cells (CHO-K1) and, when loaded with MTX, led to enhanced cytotoxicity compared to the free drug.

Graphical abstract: Dually sensitive dextran-based micelles for methotrexate delivery

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Publication details

The article was received on 17 Jan 2017, accepted on 17 Feb 2017 and first published on 06 Mar 2017


Article type: Paper
DOI: 10.1039/C7RA00696A
Citation: RSC Adv., 2017,7, 14448-14460
  • Open access: Creative Commons BY license
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    Dually sensitive dextran-based micelles for methotrexate delivery

    B. Blanco-Fernandez, A. Concheiro, H. Makwana, F. Fernandez-Trillo, C. Alexander and C. Alvarez-Lorenzo, RSC Adv., 2017, 7, 14448
    DOI: 10.1039/C7RA00696A

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