Jump to main content
Jump to site search

Issue 9, 2017
Previous Article Next Article

How do fluorescence spectroscopy and multimodal fluorescence imaging help to dissect the enhanced efficiency of the vancomycin–rifampin combination against Staphylococcus aureus infections?

Author affiliations

Abstract

Staphylococcus aureus is one of the most frequent pathogens responsible for biofilm-associated infections. Among current clinical antibiotics, very few enable long-term successful treatment. Thus, it becomes necessary to better understand antibiotic failures and successes in treating infections in order to master the use of proper antibiotic therapies. In this context, we took benefit from a set of fluorescence spectroscopy and imaging methods, with the support of conventional microbiological tools to better understand the vancomycin–rifampin combination (in)efficiency against S. aureus biofilms. It was shown that both antibiotics interacted by forming a complex. This latter allowed a faster penetration of the drugs before dissociating from each other to interact with their respective biological targets. However, sufficiently high concentrations of free vancomycin should be maintained, either by increasing the vancomycin concentration or by applying repetitive doses of the two drugs, in order to eradicate rifampin-resistant mutants.

Graphical abstract: How do fluorescence spectroscopy and multimodal fluorescence imaging help to dissect the enhanced efficiency of the vancomycin–rifampin combination against Staphylococcus aureus infections?

Back to tab navigation

Supplementary files

Publication details

The article was received on 01 Mar 2017, accepted on 11 Jun 2017 and first published on 12 Jun 2017


Article type: Paper
DOI: 10.1039/C7PP00079K
Citation: Photochem. Photobiol. Sci., 2017,16, 1391-1399
  •   Request permissions

    How do fluorescence spectroscopy and multimodal fluorescence imaging help to dissect the enhanced efficiency of the vancomycin–rifampin combination against Staphylococcus aureus infections?

    R. Boudjemaa, R. Briandet, M. Fontaine-Aupart and K. Steenkeste, Photochem. Photobiol. Sci., 2017, 16, 1391
    DOI: 10.1039/C7PP00079K

Search articles by author

Spotlight

Advertisements