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Issue 10, 2017
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Real-time in situ analysis of biocorona formation and evolution on silica nanoparticles in defined and complex biological environments

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Abstract

Biomolecules such as proteins immediately adsorb on the surface of nanoparticles upon their exposure to a biological environment. The formed adlayer is commonly referred to as biomolecule corona (biocorona) and defines the biological activity and toxicity of the nanoparticle. Therefore, it is essential to understand in detail the biocorona formation process, and how it is governed by parameters like composition of the biological environment, and nanoparticle size, shape and faceting. Here we present a detailed equilibrium and real time in situ study of biocorona formation at SiO2-nanoparticle surfaces upon exposure to defined (BSA, IgG) and complex (bovine serum, IgG depleted bovine serum) biological samples. We use both nanofabricated surface-associated Au core–SiO2 shell nanoparticles (faceted, d = 92–167 nm) with integrated nanoplasmonic sensing function and dispersed SiO2 nanoparticles (using DLS and SDS-PAGE). The results show that preadsorbed BSA or IgG are exchanged for other proteins when exposed to bovine serum. In addition, the results show that IgG forms a biocorona with different properties at curved (edge) and flat (facet) SiO2-nanoparticle surfaces. Our study paves the way for further real time in situ investigations of the biocorona formation and evolution kinetics, as well as the role of molecular orientation in biocorona formation, on nanoparticles with surface faceting.

Graphical abstract: Real-time in situ analysis of biocorona formation and evolution on silica nanoparticles in defined and complex biological environments

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Publication details

The article was received on 12 Aug 2016, accepted on 23 Feb 2017 and first published on 23 Feb 2017


Article type: Paper
DOI: 10.1039/C6NR06399C
Citation: Nanoscale, 2017,9, 3620-3628
  • Open access: Creative Commons BY-NC license
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    Real-time in situ analysis of biocorona formation and evolution on silica nanoparticles in defined and complex biological environments

    R. Frost, C. Langhammer and T. Cedervall, Nanoscale, 2017, 9, 3620
    DOI: 10.1039/C6NR06399C

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