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Issue 3, 2017
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Visual detection of multiple genetically modified organisms in a capillary array

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Abstract

There is an urgent need for rapid, low-cost multiplex methodologies for the monitoring of genetically modified organisms (GMOs). Here, we report a [C with combining low line]apillary [A with combining low line]rray-based [L with combining low line]oop-mediated isothermal amplification for [M with combining low line]ultiplex visual detection of nucleic acids (CALM) platform for the simple and rapid monitoring of GMOs. In CALM, loop-mediated isothermal amplification (LAMP) primer sets are pre-fixed to the inner surface of capillaries. The surface of the capillary array is hydrophobic while the capillaries are hydrophilic, enabling the simultaneous loading and separation of the LAMP reaction mixtures into each capillary by capillary forces. LAMP reactions in the capillaries are then performed in parallel, and the results are visually detected by illumination with a hand-held UV device. Using CALM, we successfully detected seven frequently used transgenic genes/elements and five plant endogenous reference genes with high specificity and sensitivity. Moreover, we found that measurements of real-world blind samples by CALM are consistent with results obtained by independent real-time PCRs. Thus, with an ability to detect multiple nucleic acids in a single easy-to-operate test, we believe that CALM will become a widely applied technology in GMO monitoring.

Graphical abstract: Visual detection of multiple genetically modified organisms in a capillary array

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Publication details

The article was received on 27 Oct 2016, accepted on 06 Jan 2017 and first published on 06 Jan 2017


Article type: Paper
DOI: 10.1039/C6LC01330A
Citation: Lab Chip, 2017,17, 521-529
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    Visual detection of multiple genetically modified organisms in a capillary array

    N. Shao, J. Chen, J. Hu, R. Li, D. Zhang, S. Guo, J. Hui, P. Liu, L. Yang and S. Tao, Lab Chip, 2017, 17, 521
    DOI: 10.1039/C6LC01330A

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