Jump to main content
Jump to site search

Issue 6, 2017
Previous Article Next Article

A low-cost smartphone-based platform for highly sensitive point-of-care testing with persistent luminescent phosphors

Author affiliations

Abstract

Through their computational power and connectivity, smartphones are poised to rapidly expand telemedicine and transform healthcare by enabling better personal health monitoring and rapid diagnostics. Recently, a variety of platforms have been developed to enable smartphone-based point-of-care testing using imaging-based readout with the smartphone camera as the detector. Fluorescent reporters have been shown to improve the sensitivity of assays over colorimetric labels, but fluorescence readout necessitates incorporating optical hardware into the detection system, adding to the cost and complexity of the device. Here we present a simple, low-cost smartphone-based detection platform for highly sensitive luminescence imaging readout of point-of-care tests run with persistent luminescent phosphors as reporters. The extremely bright and long-lived emission of persistent phosphors allows sensitive analyte detection with a smartphone by a facile time-gated imaging strategy. Phosphors are first briefly excited with the phone's camera flash, followed by switching off the flash, and subsequent imaging of phosphor luminescence with the camera. Using this approach, we demonstrate detection of human chorionic gonadotropin using a lateral flow assay and the smartphone platform with strontium aluminate nanoparticles as reporters, giving a detection limit of ≈45 pg mL−1 (1.2 pM) in buffer. Time-gated imaging on a smartphone can be readily adapted for sensitive and potentially quantitative testing using other point-of-care formats, and is workable with a variety of persistent luminescent materials.

Graphical abstract: A low-cost smartphone-based platform for highly sensitive point-of-care testing with persistent luminescent phosphors

Back to tab navigation

Supplementary files

Publication details

The article was received on 16 Sep 2016, accepted on 20 Jan 2017 and first published on 03 Feb 2017


Article type: Paper
DOI: 10.1039/C6LC01167E
Citation: Lab Chip, 2017,17, 1051-1059
  •   Request permissions

    A low-cost smartphone-based platform for highly sensitive point-of-care testing with persistent luminescent phosphors

    A. S. Paterson, B. Raja, V. Mandadi, B. Townsend, M. Lee, A. Buell, B. Vu, J. Brgoch and R. C. Willson, Lab Chip, 2017, 17, 1051
    DOI: 10.1039/C6LC01167E

Search articles by author

Spotlight

Advertisements