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Issue 2, 2017
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Zinc sparks induce physiochemical changes in the egg zona pellucida that prevent polyspermy

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Abstract

During fertilization or chemically-induced egg activation, the mouse egg releases billions of zinc atoms in brief bursts known as ‘zinc sparks.’ The zona pellucida (ZP), a glycoprotein matrix surrounding the egg, is the first structure zinc ions encounter as they diffuse away from the plasma membrane. Following fertilization, the ZP undergoes changes described as ‘hardening’, which prevent multiple sperm from fertilizing the egg and thereby establish a block to polyspermy. A major event in zona hardening is cleavage of ZP2 proteins by ovastacin; however, the overall physiochemical changes contributing to zona hardening are not well understood. Using X-ray fluorescence microscopy, transmission and scanning electron microscopy, and biological function assays, we tested the hypothesis that zinc release contributes to ZP hardening. We found that the zinc content in the ZP increases by 300% following activation and that zinc exposure modulates the architecture of the ZP matrix. Importantly, zinc-induced structural changes of the ZP have a direct biological consequence; namely, they reduce the ability of sperm to bind to the ZP. These results provide a paradigm-shifting model in which fertilization-induced zinc sparks contribute to the polyspermy block by altering conformations of the ZP matrix. This adds a previously unrecognized factor, namely zinc, to the process of ZP hardening.

Graphical abstract: Zinc sparks induce physiochemical changes in the egg zona pellucida that prevent polyspermy

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Publication details

The article was received on 14 Oct 2016, accepted on 07 Jan 2017 and first published on 19 Jan 2017


Article type: Paper
DOI: 10.1039/C6IB00212A
Citation: Integr. Biol., 2017,9, 135-144
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    Zinc sparks induce physiochemical changes in the egg zona pellucida that prevent polyspermy

    E. L. Que, F. E. Duncan, A. R. Bayer, S. J. Philips, E. W. Roth, R. Bleher, S. C. Gleber, S. Vogt, T. K. Woodruff and T. V. O’Halloran, Integr. Biol., 2017, 9, 135
    DOI: 10.1039/C6IB00212A

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