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Issue 2, 2017
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CRISPR-Cas9 technology: applications in genome engineering, development of sequence-specific antimicrobials, and future prospects

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Abstract

The development of CRISPR-Cas9 technology has revolutionized our ability to edit DNA and to modulate expression levels of genes of interest, thus providing powerful tools to accelerate the precise engineering of a wide range of organisms. In addition, the CRISPR-Cas system can be harnessed to design “precision” antimicrobials that target bacterial pathogens in a DNA sequence-specific manner. This capability will enable killing of drug-resistant microbes by selectively targeting genes involved in antibiotic resistance, biofilm formation and virulence. Here, we review the origins and mechanistic basis of CRISPR-Cas systems, discuss how this technology can be leveraged to provide a range of applications in both eukaryotic and prokaryotic systems, and finish by outlining limitations and future prospects.

Graphical abstract: CRISPR-Cas9 technology: applications in genome engineering, development of sequence-specific antimicrobials, and future prospects

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Publication details

The article was received on 20 Jul 2016, accepted on 30 Nov 2016 and first published on 01 Dec 2016


Article type: Review Article
DOI: 10.1039/C6IB00140H
Citation: Integr. Biol., 2017,9, 109-122
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    CRISPR-Cas9 technology: applications in genome engineering, development of sequence-specific antimicrobials, and future prospects

    C. de la Fuente-Núñez and T. K. Lu, Integr. Biol., 2017, 9, 109
    DOI: 10.1039/C6IB00140H

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