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Issue 5, 2017
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Revisiting binding of plutonium to transferrin by CE-ICP-MS

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Abstract

Capillary electrophoresis coupled with an inductively coupled plasma mass spectrometer was applied for the first time to determine the binding constant of human transferrin (Tf) for tetravalent plutonium. The experiments were carried out in a buffer 2-(N-morpholino)ethanesulfonic acid (MES) at pH 6, 0.1 M NaCl and at a temperature of 25 °C. The nitrilotriacetate anion (NTA) used in this study prevents the hydrolysis of plutonium and is an ideal competitor with Tf for Pu, both ligands sharing comparable binding strength. The separation revealed unambiguous two peaks associated with the complex Pu(NTA)2 used as the initial species and with Pu–transferrin. Two series of independent experiments were conducted and gave the first stepwise conditional bicarbonate-free Pu–transferrin binding constant of Image ID:c6dt04336d-t1.gif. In the absence of bicarbonate the affinity of transferrin for plutonium at pH 6 is about 104 times stronger than that of iron at pH 6.7 Image ID:c6dt04336d-t2.gif.

Graphical abstract: Revisiting binding of plutonium to transferrin by CE-ICP-MS

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Publication details

The article was received on 15 Nov 2016, accepted on 08 Jan 2017 and first published on 09 Jan 2017


Article type: Paper
DOI: 10.1039/C6DT04336D
Citation: Dalton Trans., 2017,46, 1389-1396
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    Revisiting binding of plutonium to transferrin by CE-ICP-MS

    S. Sauge-Merle, D. Lemaire, R. W. Evans, C. Berthomieu and J. Aupiais, Dalton Trans., 2017, 46, 1389
    DOI: 10.1039/C6DT04336D

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