Issue 1, 2017

Enzyme-immobilized clay nanotube–chitosan membranes with sustainable biocatalytic activities

Abstract

We have developed a simple and effective strategy to prepare an enzymatic membrane by the admixing of a halloysite clay nanotube–lipase complex and a chitosan solution. The loading of 3 wt% lipase into the lumen of halloysite clay nanotubes was enhanced with an electrostatic interaction. 10 wt% of lipase–nanotubes were dispersed in an aqueous chitosan solution and cast as a coating. The enzymatic chitosan–halloysite membranes displayed increased pH, temperature stability and long-term usability for the catalytic decomposition of lipids: 90% of the initial activity was preserved at 80 °C, 70% of the functionality remained at the high pH of 9, and 85% of the activity was sustained after ten continuous cycles of use. The placement of the second antimicrobial enzyme–lysozyme at the outer surface of the clay nanotubes allowed for a synergistic increase in the activity of the composite antibacterial formulation. The lipase/lysozyme-co-immobilized clay nanotube–chitosan membranes are promising in anti-biofouling applications.

Graphical abstract: Enzyme-immobilized clay nanotube–chitosan membranes with sustainable biocatalytic activities

Article information

Article type
Paper
Submitted
31 Oct 2016
Accepted
22 Nov 2016
First published
22 Nov 2016

Phys. Chem. Chem. Phys., 2017,19, 562-567

Enzyme-immobilized clay nanotube–chitosan membranes with sustainable biocatalytic activities

J. Sun, R. Yendluri, K. Liu, Y. Guo, Y. Lvov and X. Yan, Phys. Chem. Chem. Phys., 2017, 19, 562 DOI: 10.1039/C6CP07450B

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