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Issue 93, 2017
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Direct screening for ribozyme activity in mammalian cells

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Abstract

Engineered ribozymes are powerful tools for manipulating gene expression in living cells. However, identification of active ribozymes in mammalian cells has relied on empirical assays of a limited number of arbitrarily chosen ribozymes. Here, we synthesized 376 natural and 2625 synthetic variants of pistol ribozymes, and screened for active variants directly in mammalian cells, greatly expanding the ribozyme toolbox for biological applications.

Graphical abstract: Direct screening for ribozyme activity in mammalian cells

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Publication details

The article was received on 09 Oct 2017, accepted on 01 Nov 2017 and first published on 01 Nov 2017


Article type: Communication
DOI: 10.1039/C7CC07815C
Citation: Chem. Commun., 2017,53, 12540-12543
  • Open access: Creative Commons BY-NC license
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    Direct screening for ribozyme activity in mammalian cells

    Y. Nomura, H. Chien and Y. Yokobayashi, Chem. Commun., 2017, 53, 12540
    DOI: 10.1039/C7CC07815C

    This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material and it is not used for commercial purposes.

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