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Issue 96, 2017
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Chemical synthesis and NMR spectroscopy of long stable isotope labelled RNA

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Abstract

We showcase the high potential of the 2′-cyanoethoxymethyl (CEM) methodology to synthesize RNAs with naturally occurring modified residues carrying stable isotope (SI) labels for NMR spectroscopic applications. The method was applied to synthesize RNAs with sizes ranging between 60 to 80 nucleotides. The presented approach gives the possibility to selectively modify larger RNAs (>60 nucleotides) with atom-specifically 13C/15N-labelled building blocks. The method harbors the unique potential to address structural as well as dynamic features of these RNAs with NMR spectroscopy but also using other biophysical methods, such as mass spectrometry (MS), or small angle neutron/X-ray scattering (SANS, SAXS).

Graphical abstract: Chemical synthesis and NMR spectroscopy of long stable isotope labelled RNA

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Publication details

The article was received on 29 Aug 2017, accepted on 28 Sep 2017 and first published on 20 Nov 2017


Article type: Communication
DOI: 10.1039/C7CC06747J
Citation: Chem. Commun., 2017,53, 12938-12941
  • Open access: Creative Commons BY license
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    Chemical synthesis and NMR spectroscopy of long stable isotope labelled RNA

    J. Kremser, E. Strebitzer, R. Plangger, M. A. Juen, F. Nußbaumer, H. Glasner, K. Breuker and C. Kreutz, Chem. Commun., 2017, 53, 12938
    DOI: 10.1039/C7CC06747J

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