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Issue 61, 2017
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Probing the excited-state chemical shifts and exchange parameters by nitrogen-decoupled amide proton chemical exchange saturation transfer (HNdec-CEST)

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Abstract

CEST-NMR spectroscopy is a powerful tool for probing the conformational dynamics of macromolecules. We present a HNdec-CEST experiment that simplifies the relaxation matrix, reduces fitting parameters, and enhances signal resolution. Importantly, fitting of HNdec-CEST profiles enables robust extraction of exchange rates as well as excited-state chemical shifts and populations.

Graphical abstract: Probing the excited-state chemical shifts and exchange parameters by nitrogen-decoupled amide proton chemical exchange saturation transfer (HNdec-CEST)

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Publication details

The article was received on 29 Jun 2017, accepted on 07 Jul 2017 and first published on 07 Jul 2017


Article type: Communication
DOI: 10.1039/C7CC05021F
Citation: Chem. Commun., 2017,53, 8541-8544
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    Probing the excited-state chemical shifts and exchange parameters by nitrogen-decoupled amide proton chemical exchange saturation transfer (HNdec-CEST)

    Q. Wu, B. A. Fenton, J. L. Wojtaszek and P. Zhou, Chem. Commun., 2017, 53, 8541
    DOI: 10.1039/C7CC05021F

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