Mass spectrometry captures structural intermediates in protein fiber self-assembly

Michael Landreh; Marlene Andersson; Erik G. Marklund; Qiupin Jia; Qing Meng; Jan Johansson; Carol V. Robinson; Anna Rising

doi:10.1039/C7CC00307B

Mass spectrometry captures structural intermediates in protein fiber self-assembly†

Michael Landreh,^a Marlene Andersson,^b Erik G. Marklund,^c Qiupin Jia,^d Qing Meng,^d Jan Johansson,*^be Carol V. Robinson

*^a and Anna Rising*^be

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* Corresponding authors

^a Department of Chemistry, Physical & Theoretical Chemistry Laboratory, University of Oxford, South Parks Road, Oxford, UK
E-mail: Carol.Robinson@chem.ox.ac.uk

^b Department of Anatomy, Physiology and Biochemistry, Swedish University of Agricultural Sciences, Uppsala, Sweden

^c Department of Chemistry – BMC, Uppsala University, Box 576, SE-751 23, Uppsala, Sweden

^d Institute of Biological Sciences and Biotechnology, Donghua University, Shanghai, P. R. China

^e Division of Neurogeriatrics, Department of Neurobiology, Care Sciences and Society (NVS), Center for Alzheimer Research, Karolinska Institutet, Huddinge, 14157, Stockholm, Sweden
E-mail: Janne.Johansson@ki.se, Anna.Rising@ki.se

Abstract

Self-assembling proteins, the basis for a broad range of biological scaffolds, are challenging to study using most structural biology approaches. Here we show that mass spectrometry (MS) in combination with MD simulations captures structural features of short-lived oligomeric intermediates in spider silk formation, providing direct insights into its complex assembly process.

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DOI: https://doi.org/10.1039/C7CC00307B
Article type: Communication
Submitted: 13 Jan 2017
Accepted: 01 Feb 2017
First published: 01 Feb 2017
This article is Open Access

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Chem. Commun., 2017,53, 3319-3322

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Mass spectrometry captures structural intermediates in protein fiber self-assembly

M. Landreh, M. Andersson, E. G. Marklund, Q. Jia, Q. Meng, J. Johansson, C. V. Robinson and A. Rising, Chem. Commun., 2017, 53, 3319 DOI: 10.1039/C7CC00307B

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. You can use material from this article in other publications without requesting further permissions from the RSC, provided that the correct acknowledgement is given.

Chemical Communications

Mass spectrometry captures structural intermediates in protein fiber self-assembly†

Abstract

Supplementary files

Article information

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Mass spectrometry captures structural intermediates in protein fiber self-assembly

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