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Issue 1, 2017
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A platform for the development of novel biosensors by configuring allosteric transcription factor recognition with amplified luminescent proximity homogeneous assays

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Abstract

A wide range of chemicals can be sensed by allosteric transcription factors (aTFs) in bacteria. Herein, we report a biosensing platform by using isolated aTFs as recognition elements in vitro. Moreover, a general strategy to increase the sensitivity of the aTF-based biosensors is provided. As a proof-of-concept, we obtained by far the most sensitive uric acid and oxytetracycline biosensors by using aTF HucR and OtrR as recognition elements, respectively. As a large number of aTFs are present in bacteria, our work opens a novel route to develop sensitive aTF-based biosensors.

Graphical abstract: A platform for the development of novel biosensors by configuring allosteric transcription factor recognition with amplified luminescent proximity homogeneous assays

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Publication details

The article was received on 05 Sep 2016, accepted on 15 Nov 2016 and first published on 15 Nov 2016


Article type: Communication
DOI: 10.1039/C6CC07244E
Citation: Chem. Commun., 2017,53, 99-102
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    A platform for the development of novel biosensors by configuring allosteric transcription factor recognition with amplified luminescent proximity homogeneous assays

    S. Li, L. Zhou, Y. Yao, K. Fan, Z. Li, L. Zhang, W. Wang and K. Yang, Chem. Commun., 2017, 53, 99
    DOI: 10.1039/C6CC07244E

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