Jump to main content
Jump to site search


Optimizing sampling strategies for NMR-based metabolomics of human feces: Pooled vs Unpooled analyses

Abstract

This study focused on elucidating fecal sample collection for 1H NMR-based metabolite profiling. Pooled vs unpooled feces sampling was compared to determine if single, unpooled samples are adequate and representative for NMR-based metabolomics of feces. Our results show that the metabolome of unpooled fecal samples varied within the individual (day-to-day variation). This variation could mainly be ascribed to fluctuations (CV > 40%) in the level of the metabolites glucose, lactate, and succinate. Consequently, our data suggest that multiple sampling and pooling of fecal samples could be considered to minimize errors arising from day-to-day variation in fecal samples.

Back to tab navigation

Supplementary files

Publication details

The article was received on 13 Jun 2017, accepted on 14 Jul 2017 and first published on 17 Jul 2017


Article type: Technical Note
DOI: 10.1039/C7AY01465A
Citation: Anal. Methods, 2017, Accepted Manuscript
  •   Request permissions

    Optimizing sampling strategies for NMR-based metabolomics of human feces: Pooled vs Unpooled analyses

    S. Lamichhane, U. K. Sundekilde, T. Blædel, T. K. Dalsgaard, L. H. Larsen, L. O. Dragsted, A. Astrup and H. C. Bertram, Anal. Methods, 2017, Accepted Manuscript , DOI: 10.1039/C7AY01465A

Search articles by author

Spotlight

Advertisements